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Table 2 Effect of N-thiocarboxyanhydrides (NTAs) on boar sperm motility and kinetics under H2O2-induced oxidative stress

From: N-thiocarboxyanhydrides, amino acid-derived enzyme-activated H2S donors, enhance sperm mitochondrial activity in presence and absence of oxidative stress

 

Conc. (nM)

Total motility (%)

Progressive motility (%)

VAP (μm/s)

VCL (μm/s)

VSL (μm/s)

ALH (μm)

BCF (Hz)

Control

 

78.07 ± 4.35a

74.61 ± 3.63a

89.21 ± 6.92a

122.21 ± 16.38a

84.01 ± 5.86a

4.89 ± 0.51a

18.98 ± 0.73a

Control-ox

 

58.63 ± 6.75b

68.47 ± 7.02ab

42.78 ± 12.60b

67.22 ± 15.91b

41.25 ± 12.30b

2.68 ± 0.69b

15.62 ± 0.82b

Gly-NTA

10

63.54 ± 7.61b

71.00 ± 4.92ab

43.23 ± 13.34b

68.04 ± 17.51b

41.64 ± 13.12b

2.71 ± 0.80b

15.49 ± 1.32b

 

1

58.25 ± 12.93b

65.78 ± 11.54b

44.72 ± 13.99b

70.00 ± 18.21b

43.05 ± 13.69b

2.79 ± 0.81b

15.29 ± 1.55b

Leu-NTA

10

60.69 ± 8.01b

69.39 ± 4.88ab

39.98 ± 12.66b

62.18 ± 15.64b

38.46 ± 12.30b

2.48 ± 0.68b

15.77 ± 1.41b

 

1

62.10 ± 7.83b

69.26 ± 5.08ab

40.18 ± 11.45b

62.96 ± 14.56b

38.77 ± 11.36b

2.53 ± 0.64b

15.52 ± 1.07b

  1. Samples were analyzed after 1.5 h incubation at 38 °C. Oxidative stress was induced by exposing sperm cells to H2O2 10 μM. Different letters within the same column indicate statistically significant differences among treatments (p < 0.05). ALH Amplitude of lateral head displacement, BFC Beat-cross frequency, Conc. Concentration, Gly-NTA Glycine conjugated with NTA, Leu-NTA Leucine conjugated with NTA, Ox oxidative stress, VAP Average path velocity, VCL Curvilinear velocity, VSL Straight-line velocity. Data are shown as mean ± SD of six replicates