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Table 1 Effect of N-thiocarboxyanhydrides (NTAs) on boar sperm motility and kinetics during 3.5 h incubation at 38 °C

From: N-thiocarboxyanhydrides, amino acid-derived enzyme-activated H2S donors, enhance sperm mitochondrial activity in presence and absence of oxidative stress

Time

 

Conc. (nM)

Total motility (%)

Progressive motility (%)

VAP (μm/s)

VCL (μm/s)

VSL (μm/s)

ALH (μm)

BCF (Hz)

1 h

Control

 

64.94 ± 9.06

63.27 ± 6.92b

85.86 ± 19.29a

120.94 ± 32.29a

80.00 ± 16.07a

4.92 ± 1.13a

18.35 ± 1.62

Gly-NTA

10

68.10 ± 5.54

67.58 ± 4.60

87.06 ± 17.64

124.97 ± 30.75

80.97 ± 15.43

5.08 ± 1.12

17.47 ± 0.98

 

1

69.54 ± 6.59

67.56 ± 6.85

85.17 ± 14.78

122.93 ± 26.66

79.39 ± 12.40

5.00 ± 1.05

17.78 ± 1.30

Leu-NTA

10

68.72 ± 6.72

66.04 ± 4.97

86.37 ± 18.73

123.58 ± 31.64

80.30 ± 15.79

4.98 ± 1.13

18.41 ± 1.79

 

1

69.29 ± 4.58

66.78 ± 7.47

84.53 ± 17.67

122.47 ± 32.17

77.97 ± 15.35

4.91 ± 1.09

18.12 ± 1.37

3.5 h

Control

 

65.21 ± 10.34

67.98 ± 8.99ab

70.91 ± 12.84ab

95.41 ± 20.58ab

67.69 ± 11.36ab

3.90 ± 0.73b

19.57 ± 0.70

Gly-NTA

10

69.62 ± 6.15

72.34 ± 5.48a

68.05 ± 18.20b

92.51 ± 26.50b

65.16 ± 17.19b

3.79 ± 1.02b

18.65 ± 1.84

 

1

67.72 ± 5.77

70.35 ± 6.91a

72.04 ± 12.10ab

97.20 ± 21.18ab

69.26 ± 11.04ab

4.00 ± 0.68ab

19.16 ± 1.13

Leu-NTA

10

67.95 ± 3.39

71.51 ± 3.49a

67.22 ± 13.51b

88.74 ± 20.96b

64.80 ± 12.69b

3.69 ± 0.72b

19.63 ± 0.69

 

1

67.71 ± 3.48

69.87 ± 6.27a

69.96 ± 15.32ab

93.40 ± 23.98ab

67.21 ± 13.99ab

3.86 ± 0.87b

19.43 ± 0.45

  1. No statistically significant differences were found among treatments at each incubation time. Different letters within the same column indicate statistically significant differences among treatments at 3.5 h compared to the Control group at 1 h of incubation (p < 0.05). ALH Amplitude of lateral head displacement, BFC Beat-cross frequency, Conc. Concentration, Gly-NTA Glycine conjugated with NTA, Leu-NTA Leucine conjugated with NTA, VAP Average path velocity, VCL Curvilinear velocity, VSL Straight-line velocity. Data are shown as mean ± SD of six replicates