Time | Conc. (nM) | Total motility (%) | Progressive motility (%) | VAP (μm/s) | VCL (μm/s) | VSL (μm/s) | ALH (μm) | BCF (Hz) | |
---|---|---|---|---|---|---|---|---|---|
1 h | Control | 64.94 ± 9.06 | 63.27 ± 6.92b | 85.86 ± 19.29a | 120.94 ± 32.29a | 80.00 ± 16.07a | 4.92 ± 1.13a | 18.35 ± 1.62 | |
Gly-NTA | 10 | 68.10 ± 5.54 | 67.58 ± 4.60 | 87.06 ± 17.64 | 124.97 ± 30.75 | 80.97 ± 15.43 | 5.08 ± 1.12 | 17.47 ± 0.98 | |
1 | 69.54 ± 6.59 | 67.56 ± 6.85 | 85.17 ± 14.78 | 122.93 ± 26.66 | 79.39 ± 12.40 | 5.00 ± 1.05 | 17.78 ± 1.30 | ||
Leu-NTA | 10 | 68.72 ± 6.72 | 66.04 ± 4.97 | 86.37 ± 18.73 | 123.58 ± 31.64 | 80.30 ± 15.79 | 4.98 ± 1.13 | 18.41 ± 1.79 | |
1 | 69.29 ± 4.58 | 66.78 ± 7.47 | 84.53 ± 17.67 | 122.47 ± 32.17 | 77.97 ± 15.35 | 4.91 ± 1.09 | 18.12 ± 1.37 | ||
3.5 h | Control | 65.21 ± 10.34 | 67.98 ± 8.99ab | 70.91 ± 12.84ab | 95.41 ± 20.58ab | 67.69 ± 11.36ab | 3.90 ± 0.73b | 19.57 ± 0.70 | |
Gly-NTA | 10 | 69.62 ± 6.15 | 72.34 ± 5.48a | 68.05 ± 18.20b | 92.51 ± 26.50b | 65.16 ± 17.19b | 3.79 ± 1.02b | 18.65 ± 1.84 | |
1 | 67.72 ± 5.77 | 70.35 ± 6.91a | 72.04 ± 12.10ab | 97.20 ± 21.18ab | 69.26 ± 11.04ab | 4.00 ± 0.68ab | 19.16 ± 1.13 | ||
Leu-NTA | 10 | 67.95 ± 3.39 | 71.51 ± 3.49a | 67.22 ± 13.51b | 88.74 ± 20.96b | 64.80 ± 12.69b | 3.69 ± 0.72b | 19.63 ± 0.69 | |
1 | 67.71 ± 3.48 | 69.87 ± 6.27a | 69.96 ± 15.32ab | 93.40 ± 23.98ab | 67.21 ± 13.99ab | 3.86 ± 0.87b | 19.43 ± 0.45 |