A blend of functional amino acids and grape polyphenols improves the pig capacity to cope with an inflammatory challenge caused by poor hygiene of housing conditions

Table 4 Blood and plasma concentrations of indicators of inflammation and oxidative status in CNT and AAP pigs housed in good (good) or poor (poor) hygiene of housing conditions for 3 weeks¹

Experimental diets²	CNT		AAP		SEM⁴	P-value⁵
Hygiene conditions³	Good	Poor	Good	Poor	SEM⁴	ED	Hyg	ED × Hyg
No.⁶	20	20	20	20
Week 9
WBC, m/mm³	18.51	21.49	19.56	21.85	0.48	0.34	< 0.01	0.81
Granulocyte, m/mm³	5.77	8.27	6.77	9.05	0.25	0.14	< 0.01	0.47
Vitamin A, µmol/L	0.49	0.48	0.49	0.41	0.02	0.43	0.16	0.24
Diamine Oxidase, pg/ml	310.8	333.4	321.9	315.5	9.06	0.81	0.57	0.31
dROM, CARRU⁷	1035	1150	1036	1148	18.6	0.97	< 0.01	0.98
BAP, µmol/L	2823	2836	2707	2800	26.1	0.15	0.26	0.37

WBC White Blood Cells, dROM Diacron Reactive Oxygen Metabolites, BAP Biological Antioxidant Potential
¹Values are least square means
²Pigs were fed the CNT or AAP diet for 6 weeks after weaning (Week 0 to 6). The AAP diet consisted of a control standard diet (CNT) supplemented with 0.2% of a blend of functional amino acids (L-arginine, L-cystine, L-leucine, L-valine, L-isoleucine, and L-glutamine) and grape polyphenols
³Pigs were housed in good (good) and poor (poor) hygiene of housing conditions for 3 weeks (from week 7 to 9) and were fed the same standard diet
⁴Standard error of the mean
⁵Probability values for the effect of experimental diets (ED), hygiene conditions (Hyg) and their interaction (ED × Hyg)
⁶Number of pigs per group
⁷CARRU (Carratelli Unit, 1 CARRU = 0.08 mg H₂O₂/100 mL of sample)