Fig. 10

Nude mouse tumorigenicity assay. A The primary tumor appeared as a small solid mass at the injection site. Diffuse to coalescing whitish lesions (white arrow) were noted on the diaphragm. B Tumor volume of the nude mice. The data are shown as the mean ± SD, n = 10, (C) Epithelial cells formed nests and irregular tubules on a spindoloid stromal proliferation in a primary xenograft tumor. Scale bar = 100 μm (indicated in the upper left corner of the image). D Diaphragmatic muscle degeneration and atrophy adjacent to the neoplastic cell infiltrates. Scale bar = 50 μm (indicated in the upper left corner of the image)