Detection and molecular characterization of Clostridium perfringens, Paeniclostridium sordellii and Clostridium septicum from lambs and goat kids with hemorrhagic abomasitis in Turkey

Table 2 DNA sequences of primers used in this study

Target gene	Sequence (5’-3’)	Amplicon size (bp)	References
C. perfringens
cpa	GTTGTAAGCGCAGGACATGTTAAG CATGTAGTCATCTGTTCCAGCATC	402	[51]
cpb	GCGAATATGCTGAATCATCTA GCAGGAACATTAGTATATCTTC	196	[52]
etx	CGGGTGATATCCATCTATTC CCACTTACTTGTCCTACTAAC	655	[52]
itx	AAACGCATTAAAGCTCACACC GTGCATAACCTGGAATGGCT	293	[52]
cpe	GGGGAACCCTCAGTAGTTTCA ACCAGCTGGATTTGAGTTTAATG	506	[53]
cpb2	AGATTTTAAATATGATCCTAACC CAATACCCTTCACCAAATACTC	567	[54]
netB	GCTGGTGCTGGAATAAATGC TCGCCATTGAGTAGTTTCCC	384	[55]
P. sordellii
sdlO	TTACAGTTCAAAACCCAACCTATGG TGCAGCTTGTACATCTTTGCTCTTA	470	[24]
tcsl	AGAATGTGAGATAAATGTTGCTTCA ATCCTAAATCCATTTTCAGTCTTGG	228	[23]
tcsh	ATTGTGGCACGAGCTTCTGG TCCAGCTATAGAATTAGGTGGCA	153	[23]
C. septicum
Alpha toxin	AATTCAGTGTGCGGCAGTAG CCTGCCCCAACTTCTCTTTT	270	[27]
fliC	AGAATAAACAGAAGCTGGAGATG TTTATTGAATTGTGTTTGTGAAG	294	[27]

ISSN: 1746-6148