Table 2 Calculation the melting temperature in the qRT-PCR amplified region of all the PPRV lineages II and IV strains
Foreign Strains (Lineage II) | Accession no. | Melting Temp. ℃ | Foreign Strains (Lineage IV) | Accession no. | Melting Temp. ℃ | Chinese Strains (Lineage IV) | Accession no. | Melting Temp. ℃ | Chinese Strains (Lineage IV) | Accession no. | Melting Temp. ℃ |
|---|---|---|---|---|---|---|---|---|---|---|---|
Nigeria/75/1 | X74443 | 82.49 | Izatnagar/94 | KR140086 | 81.19 | China/Tibet/0701 | EU360596 | 80.82 | CH/HNZK/2014 | KM089831 | 80.82 |
Nigeria/75/1cells | HQ197753 | 82.49 | Sungri/96 | KF727981 | 81.19 | China/Tib/07 | JF939201 | 80.82 | ChinaSC2014 | MF443338 | 81.0 |
Sierra Leone/048/2011 | MF741712 | 82.3 | Sungri 1996 MSD | KJ867542 | 81.19 | China/33/2007 | KX421388 | 80.82 | ChinaNX2014 | MF443340 | 81.0 |
CIV/01/P/2009 | KR781451 | 82.67 | Israel-2536/Hebron/1997 | OL310687 | 81.38 | China/Tibet/Bharal/2008 | JX217850 | 80.82 | ChinaLN2014 | MF443341 | 81.0 |
GhanaNK1 2010 | KJ466104 | 82.49 | Turkey 2000 | NC-006383 | 81.19 | China/XJ4/2013 | KX421386 | 81.0 | ChinaJS2014 | MF443343 | 81.0 |
Benin/B1/1969 | KR781450 | 82.12 | Bangladesh/BD2/2008 | MG581412 | 80.82 | China/XJ5/2013 | KX421387 | 81.0 | ChinaHeN2014 | MF443347 | 81.0 |
Lib/2015 | KU236379 | 82.30 | Morocco 2008 | KC594074 | 81.19 | China/XJYL/2013 | KM091959 | 81.0 | ChinaGZ2014 | MF443349 | 81.0 |
Benin/10/2011 | KR781449 | 82.12 | Ethiopia 2010 | KJ867541 | 81.0 | China/XJ3/2013 | KX421385 | 81.0 | ChinaGS2014 | MF443351 | 81.0 |
SnDk11I13 | KM212177 | 82.12 | Kurdistan/2011 | MK408669 | 81.19 | China/XJ2/2013 | KX421384 | 81.0 | ChinaGD2014 | MF443352 | 81.0 |
| Â | Â | Â | DRC/Tshela/27/2012 | OL310685 | 80.82 | ChinaAH2014 | MF443354 | 81.0 | ChinaYN2014 | MF443336 | 81.0 |
| Â | Â | Â | NGYO2013-2162 | KR828813 | 80.82 | ChinaHB2014 | MF443348 | 81.0 | ChinaSX2014 | MF443337 | 81.0 |
| Â | Â | Â | India TN Gingee 2014 | KR261605 | 80.82 | ChinaCQ2014 | MF443353 | 81.0 | ChinaZJ2014 | MF443335 | 81.0 |
| Â | Â | Â | Israel/1571/Um-El-Fahem/2014 | OL310704 | 81.19 | ChinaHN2014 | MF443345 | 81.0 | Â | Â | Â |
| Â | Â | Â | IND/TN/GIN/2014/01 | KT270355 | 80.82 | ChinaJX2014 | MF443342 | 81.0 | Â | Â | Â |
| Â | Â | Â | IND/TN/VM/2014/02 | KT860063 | 80.82 | CH/HNZM/2014 | KM089832 | 80.82 | Â | Â | Â |
| Â | Â | Â | IND/TN/VEL/2015/03 | KT860064 | 80.63 | ChinaJL2014 | MF443344 | 81.0 | Â | Â | Â |
| Â | Â | Â | IND/TN/ED/2015/04 | KT860065 | 80.63 | ChinaSaX2014 | MF443339 | 81.0 | Â | Â | Â |
| Â | Â | Â | S15 | KY885100 | 81.19 | ChinaHLJ2014 | MF443346 | 81.0 | Â | Â | Â |
| Â | Â | Â | Georgia/Tbilisi/2016 | MF737202 | 80.82 | CH/HNNY/2014 | KM089830 | 81.0 | Â | Â | Â |
| Â | Â | Â | PPRV/Mongolia/9/2016 | KY888168 | 80.82 | ChinaGX2014 | MF443350 | 81.0 | Â | Â | Â |
| Â | Â | Â | IND/Delhi/2016/05 | KX033350 | 80.82 | GZL-14 | KM816619 | 81.0 | Â | Â | Â |
| Â | Â | Â | 1008 | MF678816 | 80.82 | CH/GDDG/2014 | KP868655 | 81.0 | Â | Â | Â |
| Â | Â | Â | Siberian-ibex/Mongolia/2017-01 | MZ061721 | 81.0 | China/BJ/2014 | KP260624 | 81.0 | Â | Â | Â |
| Â | Â | Â | Turkey/Central-Anatolia/2018 | MN657232 | 81.0 | China/XJBZ/2015 | KT633939 | 81.0 | Â | Â | Â |
| Â | Â | Â | Mongolia/2017-01 | MZ061722 | 81.0 | PPRV-FY | KX354359 | 81.0 | Â | Â | Â |