Fig. 3From: Simultaneous detection and identification of Peste des petits ruminants Virus Lineages II and IV by MCA-Based real-time quantitative RT-PCR assay within single reactionSpecificity of the real-time qRT-PCR assay. The specific fluorescent signals were detected from cDNA of PPRV lineage II and PPRV lineage IV plasmids, and the dissociation curves showed that there were two specific product peaks for PPRV lineages II and IV, respectively, but no specific amplification for negative control (ORFV, GTPV, and FMDV)Back to article page