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Fig. 5 | BMC Veterinary Research

Fig. 5

From: NADH oxidase of Mycoplasma synoviae is a potential diagnostic antigen, plasminogen/fibronectin binding protein and a putative adhesin

Fig. 5

Adherence and inhibition of MS or MG to DF-1 cells. MS WVU1853, MS JS1, MS SD1, and MG Rlow were pre-incubated with rabbit anti-rMSNOX, anti-MS (for MS isolates), anti-MG (for MG Rlow), or pre-immune serum, which were then allowed to adhere to DF-1 cells. In indirect immunofluorescence assays (A), the adhered mycoplasma were labeled with goat anti-rabbit IgG-FITC (green), and the cell membranes and nuclei were stained with Dil (red) and DAPI (blue), respectively. B The FI ratios of FITC to DAPI for adhered MS or MG bacteria were assessed by ImageJ software. In colony counting assays (C), the CFUs of adhered MS or MG bacteria with each treatment were assessed through colony counting. Significant differences were analyzed with two-way ANOVA in GraphPad prism 6

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BMC Veterinary Research

ISSN: 1746-6148

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