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Fig. 4 | BMC Veterinary Research

Fig. 4

From: NADH oxidase of Mycoplasma synoviae is a potential diagnostic antigen, plasminogen/fibronectin binding protein and a putative adhesin

Fig. 4

Adherence and inhibition of rMSNOX to DF-1 cells. A Adherence of rMSNOX to DF-1 cells were detected by indirect immunofluorescence assays. rMSNOX protein was pre-treated with rabbit anti-rMSNOX, anti-MG, or pre-immune serum, then allowed to adhere to DF-1 cells. rMSFBA protein (His-tagged cytoplasmic protein control) and DMEM (blank control) treated DF-1 cells were also detected. Both rMSNOX and rMSFBA protein were labeled with FITC-conjugated antibodies (green). The DF-1 cell membranes and nuclei were stained with Dil (red) and DAPI (blue). B The fluorescence intensity (FI) ratios of FITC (green) to DAPI (blue) for NOX adherence were assessed by ImageJ software. Significant differences were analyzed with one-way ANOVA in GraphPad prism 6

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