Fig. 4

PTV-2 VP2 multiple sequence alignment and the tertiary structure prediction. (A) Unique mutations and mutant clusters in the VP2 coding region of HeNZ1 comparing with other genotype 2 isolates. Comparing to the other PTV-2 strains from China, 12 unique amino acid mutations in HeNZ1 VP2 were recognized and labeled with orange frame, of which 11 aa residues are unique in HeNZ1 comparing with all other PTV-2 strains and were shown in red font. Two mutant clusters were indicated by blue background. The PTV-2 strain name list were covered with different colors: the yellow background are the strains identified from Europe and the strains covered with purple background were identified in China. Major epitopes of PTV VP2 were identified by Disco Tope. EF-loop in HeNZ1 VP2 were recognized and labeled with green frame. (B) The structure of PTV VP2 were predicted using the I-TASSER online tool. Unique mutations were depicted by VMD (1.9.4a48) and the surface residues of unique mutations were highlighted