Fig. 3

PTV-2 VP1 multiple sequence alignment and the tertiary structure prediction. (A) Unique mutations and mutant clusters in the VP1 coding region of HeNZ1 comparing with other genotype 2 isolates. Comparing to the other PTV-2 strains from China, 24 unique amino acid mutations in HeNZ1 VP1 were recognized and labeled with orange frame, of which 7 aa residues are unique in HeNZ1 comparing with all other PTV-2 strains and were shown in red font. Three mutant clusters are indicated by blue background. The strain name list was covered with different colors: the yellow background are the strains identified from Europe and the strains covered with purple background were identified in China. Major epitopes of PTV VP1 were identified by Disco Tope and labeled on the top of alignment. GH-loop and C terminus epitope in HeNZ1 VP1 were labeled with green frame and blue frame. (B) The structure of PTV VP1 were predicted using the I-TASSER online tool. Unique mutations were depicted by VMD (1.9.4a48) and the surface residues of unique mutations were highlighted