Genotypic and phenotypic diversity of Prototheca spp. recovered from bovine mastitis in terms of antimicrobial resistance and biofilm formation ability

Table 1 Name, sequence, amplicon size and specificity of all primers used in the study [20]

Primer name	Primer sequence. (5` 3`)	Amplicon size (bp)	Specificity
N476-F	TCGGAGTTAGCTGGTTCTCC	216	All Prototheca spp.
N476-R	ATTTTGGGGCCTTAACTGGT	216	All Prototheca spp.
N2-F	TGTAATAGATATTAGAAACGCAACAAA	508	P. zopfii genotype 2
N2-R	GCAGCAGTAGGGAATTTTGG	508	P. zopfii genotype 2 and P. blaschkeae
Bl2-F	CTTCGCCTTTGGCCTTCT	379^a	P. blaschkeae
Wk3-F	CGGGAATCTTCGGATCATTA	115	P. wickerhamii
Wk5-R	GGTCAAATGCTTAAAGGCGTA	115	P. wickerhamii

^aThe 379 bp amplicon size is the product of combination of primers Bl2-F and N2-R

ISSN: 1746-6148