Table 1 Primer sets used in molecular characterization of P. glacincola isolates
Genes | Primers sequence (5'‐3') | PCR conditions | Product sizes/ (bp) |
|---|---|---|---|
16S rRNA | F27 AGAGTTTGATCMTGGCTCAGTTGTCCGGGTTGTACTCGTC 1492R GGTTACCTTGTTACGACTT | Initial denaturation 95 °C /5 min.35 cycles of denaturation at 94 °C/60 s Annealing at 55°C/1min extension at 72°C/1.5 min | 1500 |