Fig. 6
Effect of piperine on CFTR-mediated fluid secretion in forskolin-induced swelling assay. 3D porcine duodenal enteroids were seeded on 48-well plate. Cells were then treated with piperine, CFTRinh-172, GlyH-101, or DIDS for 1 hour prior to stimulate with 5 μM forskolin. (Left panel) Representative images of 3D porcine duodenal enteroids after 3 hours treated with 5 μM forskolin (Right panel) Quantitative data of enteroid area after 3 hours treated with 5 μM forskolin. Enteroids areas were measured at least 10 enteroids per 1 sample groups (n = 5) (****: p-value < 0.0001 compared with vehicle, #: p-value < 0.05, ###: p-value < 0.001 compared with 5 μM forskolin)