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Table 2 Information on the nine cats with positive results in the protein-A/G-based ELISA, detecting anti-S1-protein IgG.

From: Guest edited collection serological study of SARS-CoV-2 antibodies in japanese cats using protein-A/G-based ELISA

Sample

No.a

Sampling date (2020)

Sample type

Age

Breed

Sexb

Living environ

-ment

Multiple cats owned

Clinical sign

Protein-A/G-based ELISA targeted:

Neutralizing Ab titre

High fever c

Respir

d

GI e

S1 (A 492 )

RBD (A 492 )

â—‹1

11th July

Serum

9y11mo

Mixed

M

Indoor-only

-

-

-

-

1.25

2.65

1:80

2

21st June

Serum

2y5mo

Somali

MC

Indoor-only

-

-

-

-

1.15

0.06

< 1:20

3

19th June

Serum

8y9mo

Mixed

MC

Indoor-only

-

-

-

-

1.12

0.25

< 1:20

4

29th July

Serum

10y3mo

Mixed

FS

Indoor-only

-

-

-

-

1.02

2.78

< 1:20

5

1st July

Serum

9mo

Bengal

F

Indoor-only

-

-

-

-

0.98

2.70

< 1:20

6

19th July

Plasma

7mo

Mixed

FS

Indoor-only

-

+

-

-

0.91

0.09

< 1:20

7

18th July

Serum

1y11mo

Mixed

F

Indoor-only

-

-

-

-

0.76

0.19

< 1:20

8

17th July

Plasma

10y4mo

Mixed

F

Free-access-outside

-

-

+

-

0.73

0.20

< 1:20

9

4th July

Serum

8y5mo

Munch-kin

MC

Indoor-only

-

-

-

-

0.57

1.17

< 1:20

  1. aSample No. 1 contained neutralizing antibody to SARS-CoV-2, which is shown as a circle. Sample Nos. 1–4 showed positive results in the ELISA detecting anti-RBD-protein antibody, which are underlined.
  2. bMC Male castrated, FS Female spayed.
  3. cHigh fever was defined as a body temperature above 39 °C.
  4. dRespiratory symptoms included cough, sneezing, conjunctivitis, and nasal and/or ocular discharge.
  5. eGI, gastrointestinal. Gastrointestinal symptoms included abdominal pain, belching, stomachache, bloating, loss of appetite, vomiting, abdominal pain, constipation, diarrhoea, and/or melena.