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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Guest edited collection serological study of SARS-CoV-2 antibodies in japanese cats using protein-A/G-based ELISA

Fig. 1

Reliability of an ELISA using protein-A/G conjugated with horseradish peroxidase (protein-A/G-based ELISA). The correlation of antibody reactivity to the S1 protein, as assessed by protein-A/G-based ELISA, with that assessed by anti-feline-IgG-based ELISA is shown. The absorbance at 492 mm (A492) was measured in both ELISAs. A492 of protein-A/G-based ELISA was normalized to positive control rabbit sera. The cut-off was set as the mean value + 3 standard deviations (SDs) of negative control samples, which is shown as a dashed line. Samples with a higher value than the cut-off value were considered positive. The regression line and 95% confidence intervals are indicated by the black line and grey area, respectively. The circles indicate values for individual tested samples. The star indicates a sample with SARS-CoV-2 neutralizing activity

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