Table 1 Determination of optimal antigen coating concentration and serum dilution by chessboard method

0.25

+

1.615

1.152

0.881

0.625

0.403

0.270

-

0.153

0.092

0.074

0.062

0.048

0.046

P/N

10.558

12.473

11.959

10.027

8.389

5.877

0.5

+

1.888

1.354

0.999

0.726

0.514

0.336

-

0.163

0.101

0.080

0.068

0.049

0.042

P/N

11.559

13.406

12.440

10.620

10.426

8.008

1

+

2.051

1.435

1.055

0.760

0.515

0.334

-

0.167

0.099

0.080

0.065

0.052

0.048

P/N

12.279

14.495

13.137

11.628

9.910

6.965

2

+

2.160

1.495

1.077

0.762

0.518

0.348

-

0.161

0.108

0.085

0.065

0.052

0.048

P/N

13.416

13.882

12.671

11.784

9.904

7.207

4

+

2.383

1.861

1.301

0.931

0.609

0.370

-

0.165

0.134

0.103

0.104

0.056

0.051

P/N

14.413

13.925

12.594

8.920

10.805

7.296