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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Establishment of indirect ELISA method for Salmonella antibody detection from ducks based on PagN protein

Fig. 2

Recombinant PagN protein expression, purification and identification. (A) Induction of expression of the PagN protein. M: Protein molecular weight marker; Lane 1: E.coil BL21(DE3) cells; Lane 2: empty vector pET-32a(+) plasmid transferred into E.coil BL21(DE3) was induced expression without IPTG; Lane 3: empty vector pET-32a(+) plasmid transferred into E.coil BL21(DE3) was induced expression by IPTG; Lane 4: PagN recombinant protein expression without IPTG induction; Lane 5: PagN recombinant protein expression by IPTG induction at 37 °C for 6 h. (B) Analysis of recombinant PagN protein expression patterns. M: Protein molecular weight marker; Lane 1: expression of the target protein in supernatant; Lane 2: expression of the target protein in inclusion bodies. (C) Analysis of the purified recombinant PagN protein by affinity chromatography. M: Protein molecular weight marker; Lane 1: purified PagN protein using nickel affinity chromatography. (D) Western blotting analysis of recombinant PagN protein. M: Protein molecular weight marker; Lane 1, 2: recombinant PagN protein (43 kDa) identified via Western blotting

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