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Fig. 6 | BMC Veterinary Research

Fig. 6

From: Evaluation of the immunogenic properties of the recombinant Histophilus somni outer membrane protein 40 kDa (rOMP40)

Fig. 6

Antibody-dependent E. coli (a) and P. multocida (b) growth inhibition. Legend: The pooled serum samples obtained from calves immunized with H. somni rOMP40 (E) and the control group (C) were tested for their activity against E. coli (6.a) and P. multocida (6.b) growth. As a positive control, FCS was used. All pools of serum and FCS were diluted at a two-fold dilution series in the range of 1:2–1:64. Bacteria (E. coli and P. multocida) were preincubated with inactivated pool of serum obtained from control/experimental calves: C0/E0- before first injection/immunization (white bar/ thin diagonal strips bar); C1/E1–3 weeks after the first injection/immunization (light gray bar/ transverse strips bar); C2/E2–2 weeks after the second injection/immunization (dark gray bar/ fat diagonal strips bar) and FCS- foetal calf serum (black bar). Measurements of bacterial growth inhibition were repeated five times. Bacterial growth inhibition was calculated using the following equation/formula: Bacterial growth rate [%] = OD600nm for bacteria preincubated with selected serum pool or FCS: OD600nm for bacteria preincubated with PBS * 100%. Significant differences between study groups are labelled with different lowercase letters (a-b) for P ≤ 0.05 and different uppercase letters (A-B) for P ≤ 0.01

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