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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Evaluation of the immunogenic properties of the recombinant Histophilus somni outer membrane protein 40 kDa (rOMP40)

Fig. 5

Immunoblotting analysis of the bacterial antigens with pooled calf serum obtained before and after immunization. Legend: a. - d. subsequent blots; Lanes 1, 6, 11, and 16: Page Ruler Prestained Protein Ladder (5 μL/lane); E.c.- Escherichia coli (3 × 107/lane); H.s.- Histophilus somni (3 × 107/lane); P.m.- Pasteurella multocida (3 × 107/lane); S.E.- Salmonella Enteritidis (3 × 107/lane); blot a.- membrane was incubated with pool of serum obtained from control calves before first injection (C0); blot b.- membrane was incubated with pool of serum obtained from control calves 2 weeks after second injection (C2); blot c.- membrane was incubated with pool of serum obtained from experimental calves before first immunization (E0); blot d.- membrane was incubated with pool of serum obtained from experimental calves 2 weeks after the second immunization (E2). As a secondary antibodies anti-bovine IgG conjugated with HRP were used. Serum pools were diluted 1:20. Arrows and frames show ~ 40 kDa antigens

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