Fig. 5From: Evaluation of the immunogenic properties of the recombinant Histophilus somni outer membrane protein 40 kDa (rOMP40)Immunoblotting analysis of the bacterial antigens with pooled calf serum obtained before and after immunization. Legend: a. - d. subsequent blots; Lanes 1, 6, 11, and 16: Page Ruler Prestained Protein Ladder (5 μL/lane); E.c.- Escherichia coli (3 × 107/lane); H.s.- Histophilus somni (3 × 107/lane); P.m.- Pasteurella multocida (3 × 107/lane); S.E.- Salmonella Enteritidis (3 × 107/lane); blot a.- membrane was incubated with pool of serum obtained from control calves before first injection (C0); blot b.- membrane was incubated with pool of serum obtained from control calves 2 weeks after second injection (C2); blot c.- membrane was incubated with pool of serum obtained from experimental calves before first immunization (E0); blot d.- membrane was incubated with pool of serum obtained from experimental calves 2 weeks after the second immunization (E2). As a secondary antibodies anti-bovine IgG conjugated with HRP were used. Serum pools were diluted 1:20. Arrows and frames show ~ 40 kDa antigensBack to article page