Fig. 3
Reactivity of IgG1 (a), IgG2 (b) and IgM (c) antibodies against rOMP40 antigen in ELISA. Legend: Figures show the determination of reactivity of IgG1 (3.a), IgG2 (3.b) and IgM (3.c) antibodies against rOMP40 antigen. The blood samples were obtained before the first immunization/injection (E0, C0), 3 weeks after first immunization/injection (E1; C1) and 2 weeks after the second immunization/injection (E2; C2). Individual study groups are labelled with C0, C1, and C2 (white bars) and E0, E1, and E2 (gray bars). Significant differences within individual study groups (C0-C2; E0-E2) are labelled with different uppercase letters (A-C) for P ≤ 0.01. Significant differences between study groups in respective dates (C0-E0; C1-E1; C2-E2) are labelled with an asterisk (*) for P ≤ 0.01. ELISA plates were coated with H. somni rOMP40 antigen (3.a-3.c). Sera were diluted 1:100