Fig. 1
H. somni rOMP40 expression using various expression strains and culture conditions. Legend: SDS-PAGE pattern of selected E. coli expression strains (3 × 107 cfu/lane) producing rOMP40 under different cultured conditions. a. - e. subsequent stained gels; A. Cells after induction of protein expression; B. Cells before induction of protein expression. C41 [C41 (DE3)], C43 [C43 (DE3)], BL21 [BL21 (DE3)], R.g. [Rosetta-gami (DE3) pLysS] – E. coli expression cells; gel a.- the rOMP40 production was induced using 0.5 or 1 mM IPTG and incubated later for 5 h at 37 °C; C41 and R.g. strains were used; gel b.- the rOMP40 production was induced using 0.5 or 1 mM IPTG and incubated later for 5 h at 37 °C; C43 and BL21 strains were used; gel c.- the rOMP40 was produced in the autoinduction process; C41, C43, BL21 and R.g. strains were used; gel d.- the rOMP40 production was induced using 0.5 mM IPTG and later incubated for 16 h at room temperature; C41, C43, BL21 and R.g. strains were used; gel e.- the rOMP40 production was induced using 1 mM IPTG and later incubated for 16 h at room temperature;; C41, C43, BL21 and R.g. strains were used. Samples were added to individual lanes 1–46. Lanes 1, 19, and 38: Spectra Multicolour Broad Range Protein Ladder (5 μL/lane); Lanes 20 and 29: Page Ruler Prestained Protein Ladder (5 μL/lane). Arrow shows 40 kDa antigen