Fig. 3

S100/calgranulin immunohistochemistry (IHC) of urinary bladder tissue biopsies in dogs with lower urinary tract disease. Upper panel: moderate numbers of infiltrating cells staining positive (Fast-red) for S100A8/A9 (A) or S100A12 (B) in a 10½-year old female mixed breed dog diagnosed with urothelial carcinoma. Lower panel: large numbers and nest-like accumulations of infiltrating cells staining positive for S100A8/A9 (C, insert at the bottom right: negative staining control) or S100A12 (D) in a 4-months old male Newfoundland dog with marked transmural suppurative cystitis. Gray scale bars at the top right corners: 100 µm