Skip to main content

Table 4 PCR Protocols for RNA and DNA virus detection

From: Detection of viruses from feces of wild endangered Macaca maura: a potential threat to moor macaque survival and for zoonotic infection

RNA Virus

PCR Protocol

References

Flavivirus

cDNA synthesis followed by PCR end point

[46], Additional file 1

Coronavirus

cDNA synthesis followed by PCR end point

[47], Additional file 1

EMCV

cDNA synthesis followed by PCR end point

[48], Additional file 1

EMCV

Real time RT-PCR

Kindly provided by dr E.A. Foglia, IZS Lombardia ed Emilia Romagna, Italy

Morbillivirus

cDNA synthesis followed by PCR end point

[49], Additional file 1

WNV Lineage 1 and 2

Real time RT-PCR

[50], Additional file 1

USUV

Real time RT-PCR

[51], Additional file 1

Influenza A virus

Real time RT-PCR

[52], Additional file 1

HAV

Real time RT-PCR

[53], Additional file 1

HEV

Real time RT-PCR

[54, 55], Additional file 1

Norovirus genogroup I (GI) and II (GII)

Real time RT-PCR

[53,54,55], Additional file 1

DNA Virus

PCR Protocol

References

Herpesvirus

nested PCR

[56], Additional file 1

Orthopoxvirus (OPV)

SYBR Green real time PCR

[57], Additional file 1

Parvovirus

Quanty® Parvo B19 kit

Additional file 1