Fig. 5
Therapeutic effect of the phage vB_SalP_LDW16 on the chicken model infected with Salmonella. A Survival rates. The chickens were intraperitoneally injected with 108 CFU/mL of S. Enteritidis S64. Two hours later, the chickens were orally fed with 106 PFU/mL of phage, amoxicillin (2.5 mg/mL), florfenicol (2 mg/mL), and neomycin (100 mg/mL). Chickens injected with bacteria, phage or saline only were set as a control group; B Dynamic changes in the bacteria in the blood in different groups. C Dynamic changes in the phage in the feces in different groups. D Histopathological images of the representative tissues and organs. The red arrow represents the lesion area. Intestine (HE, 100 ×), Heart (HE, 400 ×), Liver (HE, 400 ×), Kidney (HE, 400 ×). E The average weight of the different groups at the end of the experiment. The error bars indicate the standard deviations. Asterisks and pound signs indicate a statistically significant difference (P < 0.05), in which “#” (#p < 0.05, ##p < 0.01) are compared with the blank control group and “*” (*p < 0.05, **p < 0.01) are compared with the phage-treated group