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Table 4 Reference intervals for plasma protein electrophoresis fractions in nursehounds (Scyliorhinus stellaris), using the Sebia Minicap Protein (E) 6 Flex-Piercing.

From: Increasing the data on elasmobranch plasma protein electrophoresis: electrophoretogram reference values determination in the undulate skate (Raja Undulata) and the nursehound shark (Scyliorhinus stellaris) maintained under human care

Analyte (unit)

n

Mean

SD

Median

Min

Max

RI

LRL

URL

Distr

Method

P

TP (g/l)

62

24.66

4.22

23.50

19.00

36.00

19.58–34.28

19.00–20.00

32.00–36.00

NG

NP

< 0.01

Fraction 1 (%)

62

0.74

0.35

0.64

0.21

1.50

0.30–1.49

0.22–0.31

1.43–1.50

NG

NP

< 0.01

Fraction 2 (%)

62

1.98

0.73

1.81

0.80

3.90

0.82–3.73

0.80–1.09

3.27–3.90

NG

NP

0.017

Fraction 3 (%)

62

9.99

1.88

10.15

5.60

14.50

6.19–13.79

5.51–6.85

13.13–14.53

NG

NP

0.200

Fraction 4 (%)

62

26.62

3.46

27.16

16.60

33.68

17.61–33.58

16.60–20.40

31.39–33.68

NG

NP

0.011

Fraction 5 (%)

62

28.79

3.86

29.72

19.80

34.99

19.97–34.80

19.80-22.68

34.04–34.99

NG

NP

< 0.01

Fraction 6 (%)

62

13.22

3.16

12.69

7.68

19.66

8.06–19.65

7.68–9.21

19.21–19.66

NG

NP

0.038

Fraction 7 (%)

62

13.25

4.32

12.97

5.56

24.80

5.56–24.05

5.56–6.87

21.44–24.80

NG

NP

0.200

Fraction 8 (%)

62

5.40

1.59

5.40

1.80

8.10

2.19–8.64

1.66–2.79

8.06–9.24

NG

NP

0.200

Fraction 1 (g/l)

62

0.18

0.09

0.17

0.04

0.42

0.05–0.40

0.05–0.07

0.32–0.42

NG

NP

0.071

Fraction 2 (g/l)

62

0.49

0.21

0.43

0.19

1.17

0.22–1.03

0.19–0.25

0.89–1.17

NG

NP

0.014

Fraction 3 (g/l)

62

2.46

0.63

2.23

1.13

4.06

1.19–3.74

0.96–1.41

3.50–3.99

NG

NP

0.200

Fraction 4 (g/l)

62

6.59

1.55

6.10

4.14

10.06

4.27–9.88

4.14–4.79

9.49–10.06

NG

NP

< 0.01

Fraction 5 (g/l)

62

7.12

1.68

6.77

4.67

11.09

4.67–10.94

4.67–4.86

10.19–11.09

NG

NP

0.037

Fraction 6 (g/l)

62

3.25

0.90

3.10

1.69

5.75

1.44–5.05

1.11–1.76

4.71–5.38

NG

NP

0.200

Fraction 7 (g/l)

62

3.22

1.07

3.09

1.22

6.82

1.31–6.32

1.22–1.85

5.05–6.82

NG

NP

0.200

Fraction 8 (g/l)

62

1.35

0.51

1.29

0.52

2.57

0.32–2.37

0.14–0.52

2.17–2.55

NG

NP

0.182

Alpha-2-/ Beta-globulin ratio

62

0.67

0.12

0.67

0.35

1.06

0.50–1.09

0.50–0.52

0.89–1.29

NG

NP

< 0.01

  1. n, number of individuals; SD, standard deviation; RI, reference intervals; LRL, 90% confidence interval of the lower reference limit; URL, 90% confidence interval of the upper reference limit (90% confidence intervals of the limits determined nonparametrically used a bootstrap method); Distr, distribution; P, p-value determined using a Kolmogorov-Smirnov normality test with Lilliefors corrections (cut off value was set at 0.26); G, Gaussian; NG, non-Gaussian; P, parametric; NP, non-parametric TP, total proteins in plasma.
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BMC Veterinary Research

ISSN: 1746-6148

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