Establishment of a recombinase polymerase amplification (RPA) fluorescence assay for the detection of swine acute diarrhea syndrome coronavirus (SADS-CoV)

Table 1 Primer and probe sequences used in RT-RPA system

Names	Sequences (5’-3’)	Sizes
F-4	ATTAATAGTTTTAAGCTTTATCGCAGAACG	151 bp
R-4	CACTTAAAATCGTCAGAGTAATACCTGTT	151 bp
F-5	AAGTTTTAGAACATCTTAGAAACTGGAACT	130 bp
R-5	CACATGATTAACATCTTAACTCCATAGAGAA	130 bp
F-8	TATTACTCTGACGATTTTAAGTGGAACAC	202 bp
R-8	ATAGTCACCATGCTTACTTCTGACATAAAA	202 bp
F-10	GATTTTAAGTGGAACACTCTTTTTCGATG	191 bp
R-10	AATAGTCACCATGCTTACTTCTGACATAAA	191 bp
F-15	TTAATAGTTTTAAGCTTTATCGCAGAACG	168 bp
R-15	CATCGAAAAAGAGTGTTCCACTTAAAATC	168 bp
M-F	ATTGGTGGTTCTTCTCCTTC	316 bp
M-R	ATTATAGTCGTGCCAGGTTT	316 bp
Probes	ACGCCATTGCTGTCATTTCAGTCTTTGG[FAM-dT]A[THF]A[BHQ1-dT]CCTACTCGATACC

ISSN: 1746-6148