Fig. 3

Photomicrographs of H&E-stained Harderian gland sections of the experimental groups: A at 3dpi, CS group showing congestion with cellular and exudative cast in harderian gland lumen (H&E,100 X). RS group showing desquamation and necrosis of lining epithelium (H&E,100 X). VEC + CS, VEC + RS, CEO + CS, TCO + CS, and TCO + RS showing mild lymphoid depletion with congestion as well, while CEO + RS showing moderate lymphoid depletion (H&E,100 X). B at 7 dpi, CS group showing severe desquamation and necrosis of lining epithelium (H&E,100 X). RS group showing excessive lymphoid depletion with lymphocytolysis (H&E,200 X). VEC + CS showing mild lymphoid depletion (H&E,100 X), VEC + RS showing moderate lymphoid depletion (H&E,100 X); CEO + CS, TCO + CS groups showing moderate to severe lymphoid depletion (H&E,100 X), however CEO + RS and TCO + RS groups showing excessive lymphoid depletion (H&E,100 X). CS: CEO like strain, RS: Recombinant strain, VEC + CS: Vector vaccinated group challenged with CEO like strain, VEC + RS: Vector vaccinated group challenged with recombinant strain, CEO + CS: CEO vaccinated group challenged with CEO like strain, CEO + RS: CEO vaccinated group challenged with recombinant strain, TCO + CS: TCO vaccinated group challenged with CEO like strain, TCO + RS: TCO vaccinated group challenged with recombinant strain