Table 4 Sequences of the primers and probes for BRVA RT-PCR, real-time RT-RPA, LFS RT-RPA and real-time RT-PCR assays
Assays | Primers and probes | Sequence (5´-3´) | Amplicon size (bp) | Reference |
|---|---|---|---|---|
RT-PCR | VP6-F | ATGGATGTCCTGTACTCCTTGTC | 1177 | This study |
VP6-R | TTCTAATGGAAGCCACTGTAAATAC | |||
real-time RT-RPA | BRVA-exo-F | GTAGATAATGTATGTATGGACGAGATGGTT | 192 | This study |
BRVA-exo-R | TGGCTTATGAAATGTGAAACCCGTTCTTTG | |||
BRVA-exo-P | TTGCACCACAATCAGATTCACTCAGAAAGT(FAM-dT)(THF)(BHQ1-dT)CAGGTATTAAATTC-C3-spacer | |||
LFS RT-RPA | BRVA-nfo-F | GTAGATAATGTATGTATGGACGAGATGGTT | 192 | This study |
BRVA-nfo-R | Biotin-TGGCTTATGAAATGTGAAACCCGTT CTTTG | |||
BRVA -nfo-P | FAM-TTGCACCACAATCAGATTCACTCAGAAAGTT(THF)TCAGGTATTAAATTC-C3-spacer | |||
real-time RT-PCR | BRVA-qPCR-F | ACTCCAATGTAAGTGATCTAATTC | 138 | [18] |
BRVA-qPCR -R | GAGTTGTTCCAAGTAATCCAAA | |||
BRVA-qPCR -P | FAM-ACCAATTCCTCCAGTTTGGAAYTCATTYCC-BHQ1 |