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Table 2 Diagnostic sensitivity, diagnostic specificity, predictive value, and kappa value of BRVA real-time RT-RPA, LFB RT-RPA and real-time RT-PCR assays

From: Rapid detection of bovine rotavirus a by isothermal reverse transcription recombinase polymerase amplification assays

 

Real-time RT-PCR

 

P

N

T

Real-time RT-RPA

P

61

0

61

N

1

72

73

T

62

72

134

 

DSe:98.39%

DSp:100%

K:0.985

PPV:100%

NPV:98.63%

LFB RT-RPA

P

62

0

62

N

0

72

72

T

62

72

134

 

DSe: 100%

DSp:100%

K:1.0

PPV:100%

NPV: 100%

  1. Note: P Positive, N Negative, T Total, DSe Diagnostic sensitivity, DSp Diagnostic specificity, K Kappa coefficient value, PPV Positive predictive value, NPV Negative predictive value