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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Rapid detection of bovine rotavirus a by isothermal reverse transcription recombinase polymerase amplification assays

Fig. 3

Performance of BRVA-specific LFS RT-RPA assay. Only the genomic RNA of BRVA was amplified, and the limit of detection of the assay was 1.4 × 101 copies. A. Analytical specificity of the LFS RT-RPA assay evaluating on the common pathogens causing diarrhea in cattle. Line 1, BRVA; line 2, BVDV; line 3, BCoV; line 4, BEV; line 5, BKV; line 6, ETEC; line 7, C. perfringens; line 8, Nuclease free water. B. Analytical sensitivity of the LFS RT-RPA assay using a dilution range of 1.4 × 105 ~ 1.4 × 100 copies of BRVA standard RNA. Line 1, 1.4 × 105 copies; line 2, 1.4 × 104 copies; line 3, 1.4 × 103 copies; line 4, 1.4 × 102 copies; line 5, 1.4 × 101 copies; line 6, 1.4 × 100 copies; line 7, Nuclease free water

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