Fig. 2

Optimization of the BRVA-specific LFS RT-RPA assay. The optimal reaction condition was set to be performed at 40 °C for 20 min. A. Optimization of the reaction temperature. The target gene of BRAV was amplified successfully at 39 °C ~ 43 °C. The red bands (test lines) were visible at 39 °C ~ 43 °C, and the test line band was clearest at 40 °C, which was set as the optimal reaction temperature. B. Optimization of the reaction time. The target gene of BRVA was amplified successfully at 40 °C for 10 min ~ 30 min. The red bands (test lines) were clearer after incubation for 20 min and 30 min, and there were no clear differences for them. The optimal reaction time was set as 20 min