Fig. 4

A sandwich, double wells ELISA plate showed positive samples for Cryptosporidium antigen. Rows A, C, E, G were sensitized with the anti-Cryptosporidium-specific antibody and rows B, D, F, H coated nonspecific antibodies. Control positive added in wells A1 and B1. The diluted samples added to the wells as follows: sample 1 in wells C1 and D1, sample 2 in wells E1 and F1, sample 3 G1 and H1, etc., till sample 47 in wells G12 and H12. Validation of the test; positive control optical density net (Delta) value < 1.151. Positive samples were numbers 25, 26, 42, 44 and 47 their S/P% (Delta optical density of sample/ Delta optical density of positive control *100) were > 7%