Investigation of Toxoplasma infection in zoo animals using multispecies ELISA and GRA7 nested PCR

BMC Veterinary Research

Table 4 Primer pairs and expected PCR product size for GRA7 nPCR and B1 nPCR

Target Gene	PCR	Primer paris (5′-3′)	Expected Size of PCR product
GRA7^a	1st PCR	GRA7-FE1: CAAGCACCCGTTGACAGTCTCAG	316 bp
GRA7^a	1st PCR	GRA7-RE1: ACGATGCACCCATACCAACAGCCG	316 bp
	2nd PCR	GRA7-FI1: CACCACCAGCATGGATAAGGCATC	222 bp
	2nd PCR	GRA7-RI1: CGGCGA GCTTCTTCAGCAAGTCT	222 bp
B1^b	1st PCR	Outter-F: CTTTGAATCCCAAGCAAAACATGAG	400 bp
B1^b	1st PCR	Outter-R: GCGAGCCAAGACATCCATTGCTGA	400 bp
	2nd PCR	Inner-F: GTGATAGTATCGAAAGGTAT	220 bp
	2nd PCR	Inner-R: ACTCTCTCTCAAATGTTCCT	220 bp

ISSN: 1746-6148