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Table 1 Enrichment of nanobodies against ASFV K205R protein specific phages during three rounds of panning

From: K205R specific nanobody-horseradish peroxidase fusions as reagents of competitive ELISA to detect African swine fever virus serum antibodies

Round of panning

Input (PFU/Well)

P output (PFU/Well)

N output (PFU/Well)

Recovery rate

Enrichment(P/N)

First round

5 × 1010

1.26 × 105

5.4 × 104

2.52 × 10–6

2.3

Second round

5 × 1010

6.5 × 106

7.4 × 104

1.3 × 10–4

88

Third round

5 × 1010

7.4 × 107

6.5 × 105

1.48 × 10–3

1.14 × 102

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BMC Veterinary Research

ISSN: 1746-6148

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