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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Neural, adipocyte and hepatic differentiation potential of primary and secondary hair follicle stem cells isolated from Arbas Cashmere goats

Fig. 4

Neurogenic differentiation of HFSCs. a The first column represents the uninduced group of PHFSCs and SHFSCs (magnification × 10; scale bar, 100 µm). The second and third columns represent the differentiation of PHFSCs and SHFSCs into nerve cells (magnification × 10 and × 20; scale bar, 100 µm). b The expression of NeuN, GFAP, and MAP2 in PHFSCs and SHFSCs were determined using immunofluorescence; nuclei (blue), target proteins (green). c Western blotting to check the expression of NeuN, GFAP, and MAP2 in PHFSCs and Grey-value quantitative analysis. Full-length blots are presented in Supplementary Fig. 2. d Western blotting to check the expression of NeuN, GFAP, and MAP2 in SHFSCs and Grey-value quantitative analysis. Full-length blots are presented in Supplementary Fig. 2. e Transcript-level expression of NeuN, GFAP, and MAP2 in the induced PHFSC group. The control group comprised the uninduced PHFSCs. f Transcript-level expression of NeuN, GFAP, and MAP2 in the induced SHFSCs. The control group comprised the uninduced SHFSCs (Scale bars, 100 µm). * P < 0.05, ** P < 0.01, *** P < 0.001

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