Fig. 1

Isolation and culture of HFSCs. a Primary hair follicles (PHFs) and secondary hair follicles (SHFs) isolated from the skin of Arbas Cashmere goats. The dotted lines represent the migrated cells following culture for 1–4 days (magnification × 4; Scale bar, 500 µm). b Primary hair follicle (PHF) stem cells (PHFSCs) and secondary hair follicle (SHF) stem cells (SHFCs) purified using type IV collagen (magnification × 10 and × 20; scale bar, 100 µm). c PHFSCs and SHFSCs examined under a microscope following Giemsa staining (magnification × 10 and × 20; scale bar, 100 µm). d Growth curves of PHFSCs at the third (P3), fifth (P5), seventh (P7), and ninth (P9) passages. e Growth curves of SHFSCs at P3, P5, P7, and P9. f Viability of PHFSCs and SHFSCs at P3, P5, P7, and P9