Fig. 6

Immunofluorescence analysis (A) and quantification (B) of p65 nuclear translocation in MAC-T cells. Cells treated with LPS (1 μg/mL) were coincubated with DEX (20 μg/mL) or ISL (2.5, 5 and 10 μg/mL) for 24 h. p-p65 was labeled red with Cy3, while the nucleus was marked blue with DAPI. Values represent the means ± SEM of four independent experiments. In the above bars, ** indicate significance at P < 0.01 between the control and the LPS treatment without DEX and ISL. Among LPS in combination with DEX and ISL treatments, the same letters indicate P > 0.05; different lowercase letters indicate P < 0.05 while different uppercase letters indicate P < 0.01