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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Anti-inflammation of isoliquiritigenin via the inhibition of NF-κB and MAPK in LPS-stimulated MAC-T cells

Fig. 5

Detection of MAPK activity in MAC-T cells using Western blotting. Cells were coincubated with 1 μg/mL lipopolysaccharide (LPS) and dexamethasone (DEX) (20 μg/mL) or isoliquiritigenin (ISL) (2.5, 5 and 10 μg/mL) for 24 h. (A-F) The protein levels of ERK1/2, p38 , JNK, p-ERK, p-p38 and p-JNK were relative to those of β-actin, respectively. Values represent the means ± SEM of three independent experiments. In the above bars, ** indicate significance at P < 0.01 between the control and the LPS treatment without DEX and ISL. Among LPS in combination with DEX and ISL treatments, the same letters indicate P > 0.05; different lowercase letters indicate P < 0.05 while different uppercase letters indicate P < 0.01

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