Table 1 Primers used in this study
From: Different serotypes of Escherichia coli flagellin exert identical adjuvant effects
Gene | Primer sequence(5’-3’) |
|---|---|
fliC-F | CGCGGATCCATGGCACAAGTCATTAATACCAACAG |
fliC-R | GAGCGTCGACTTAACCCTGCAGCAGAGACAGAAC |
GAPDH-F | GCCTTCCGTGTTCCTACCC |
GAPDH-R | TGCCTGCTTCACCACCTTC |
mIl4-F | ACAGGAGAAGGGACGCCAT |
mIl4-R | GAAGCCCTACAGACGAGCTCA |
mIfng-F | TCAAGTGGCATAGATGTGGAAGAA |
mIfng-R | TGGCTCTGCAGGATTTTCATG |
mTnf-F | AGCCCCCAGTCTGTATCCTT |
mTnf -R | CTCCCTTTGCAGAACTCAGG |