Fig. 5
From: Different serotypes of Escherichia coli flagellin exert identical adjuvant effects
The mRNA level of pro-inflammatory cytokines in splenocytes. Splenocytes collected from the vaccinated mice were cultured in medium alone or the presence of FaeG protein for 48 h. A Splenocytes were analyzed with qRT-PCR for Il4 gene expression. B Splenocytes were analyzed with qRT-PCR for Tnf gene expression. C Splenocytes were analyzed with qRT-PCR for Ifng gene expression. Double asterisks indicate statistical significance over the FaeG alone immunized group (P < 0.01). Results are expressed as mean ± SD from three independent experiments. Each sample was tested in triplicate