Fig. 4

Induction of anti-FaeG IgG responses in mice. A Schematic diagram of the immunization program. Mice were immunized three times at bi-weekly intervals. Blood was collected from the caudal vein at 7, 14, 21, and 28 dpv (B) Longevity of the anti-FaeG IgG response in mice groups on different days. Data represent mean ± SD from six mice per group. C The final anti-FaeG total IgG antibody titers of mice serum after the third immunization. The antibody titers are calculated by multiplying the highest serum dilution that gave an OD reading > 0.3 after background subtraction and is presented in log₁₀. Results are expressed as mean ± standard deviation of at least three independent experiments. ** indicate the anti-FaeG IgG antibody titers over group immunized FaeG alone (P < 0.01)