Fig. 2
From: Different serotypes of Escherichia coli flagellin exert identical adjuvant effects
Extraction and detection of recombinant FliCH1, FliCH7, and FliCH19 flagellin proteins. A Coomassie blue staining of recombinant FliCH1, FliCH7, and FliCH19 proteins in SDS-PAGE. B–D The expressed FliCH1, FliCH7, and FliCH19 flagellin proteins were detected using rabbit anti-FliCH1, FliCH7, and FliCH19 (1:10,000) antiserum, respectively. HRP-labeled goat anti-rabbit IgG (1:20,000; Sigma) was used as the secondary antibodies in western blotting. Purified His6-tag proteins expressed from BL21 E. coli harboring an empty pET28α ( +) vector served as a negative control