The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa

Table 4 The functional characteristics of wisent epididymal spermatozoa cryopreserved with (Gradient FT) and without (Control FT) pre-freezing Percoll® gradient centrifugation assessed by flow cytometry. All data are presented as mean ± SEM. a,b,c, within each column, values with different superscripts are significantly different (p < 0.05)

	Cells with intact sperm membrane (%)	Cells with intact acrosome (%)	Live non apoptotic cells (%)	Cells with high mitochondrial activity (%)	Cells with damaged chromatin (%)	Cells without lipid peroxidation (%)
24 h from collection	51.46 ± 3.80^a	53.23 ± 5.55^a	54.26 ± 9.20^a	66.37 ± 5.89^a	0.14 ± 0.05^a	97.43 ± 0.89^a
Control FT	11.86 ± 3.60^b	32.23 ± 4.60^b	46.26 ± 6.17^a	20.93 ± 5.78^b	0.09 ± 0.03^a	98.15 ± 0.82^a
Gradient FT	29.93 ± 4.30^c	40.79 ± 3.80^c	46.40 ± 2.32^a	56.94 ± 4.67^c	0.07 ± 0.04^a	99.03 ± 0.34^a

ISSN: 1746-6148