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Table 2 Number of spermatozoa obtained prior and after freezing- thawing (106) from separate epididymis (L and R represent a pair of epididymides from each individual from 1 to 4)

From: The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa

 

Fresh parameters

Post thaw parameters

 

TOTAL [×106]

LIVE [×106]

MOTILE [×106]

TOTAL [×106]

LIVE [×106]

MOTILE [×106]

Control

Percoll

Control

Percoll

Control

Percoll

Control

Percoll

Control

Percoll

Control

Epididymis 1 L

728.00

410.00

607.88

342.35

364.00

225.50

728.00

129.50

83.72

53.10

14.56

Epididymis 1R

499.60

499.90

482.11

482.40

299.76

299.94

499.60

320.00

124.90

214.40

49.96

Epididymis 2 L

900.00

900.00

760.50

760.50

270.00

270.00

900.00

120.00

180.00

43.20

360.00

Epididymis 2R

900.00

860.00

722.70

690.58

360.00

344.00

900.00

232.50

342.00

156.94

360.00

Epididymis 3 L

600.00

960.00

474.00

758.40

360.00

576.00

600.00

520.00

306.00

301.60

240.00

Epididymis 3R

800.00

1960.00

632.00

1548.40

520.00

1274.00

800.00

720.00

288.00

518.40

160.00

Epididymis 4 L

160.00

1173.00

138.40

1014.65

96.00

703.80

160.00

294.00

60.80

97.20

56.00

Epididymis 4R

160.00

855.00

143.20

765.23

96.00

513.00

160.00

441.00

37.60

227.12

40.00