Fig. 1

A Amplification of COI gene produces 678 bp of PCR products from tick species. Lane M: 100 bp DNA ladder; Lane 1: DNA of field hard ticks, R. (Boophilus) annulatus, collected from Al-Behera; Lane 2, 3: DNA of field hard ticks, R. (Boophilus) annulatus, collected from Kafr El-Sheikh and Lane –ve C: Negative control, B Phylogenetic tree of R. (Boophilus) annulatus ticks in Egypt, inferred using the Maximum Composite Likelihood method on MEGA X software. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) is shown next to the branches. H. dromedarii and H. excavatum sequence was used as outgroup