Fig. 6

The fluorescence distribution of LC3. Note: MDBK cells are pretreated with 10 μM DAPT for 6 h, and then are stimulated with 5 μg/mL LPS and 25.5 mM glucose for another 24 h. LC3 was labeled with red fluorescence by Cy3, and the nucleus was labeled with blue fluorescence by DAPI. Immunofluorescence images of LC3 were shown in MDBK cells from Control, HG, LPS, HG + LPS, and HG + LPS + DAPT treatment groups. Scale bar, 10 µm