Fig. 4

Co-treatment of HG and LPS induced autophagy by Notch3-mediated mTOR signaling pathways in MDBK cells. Note: MDBK cells are pretreated with 10 μM DAPT for 6 h, and then are stimulated with 5 μg/mL LPS and 25.5 mM glucose for another 24 h. A The protein level of Notch3, mTOR, p-mTOR, LC3-II/I, p62 and Beclin1. C: control; H: HG; L: LPS; H + L: HG + LPS; H + L + DAPT: HG + LPS + DAPT. B The mRNA expression level of Notch3, mTOR, LC3B, p62 and Beclin1. *0.01 < P < 0.05 and ** P < 0.01 compared with control group; #:0.01 < P < 0.05; ##: P < 0.01