Fig. 2

Immunofluorescence observation of LC3 in MDBK cells after HG and LPS co-stimulation. Note: 0, 1, 2, 5 μg/mL LPS are co-treated with 5.5 mM glucose and 25.5 mM glucose respectively for 24 h in MDBK cells. LC3 was labeled with red fluorescence by Cy3, and the nucleus was labeled with blue fluorescence by DAPI. Scale bar, 10 µm