Table 1 Primers for gene cloning and recombinant protein construction
From: N-terminus of flagellin enhances vaccine efficacy against Actinobacillus pleuropneumoniae
Target gene | Oligonucleotide Sequence (5′ to 3′) | RE site | Gene length (bp) | NCBI Reference Sequence |
|---|---|---|---|---|
nFliC | F: GGATCCATGGCACAAGTCATTAATACAAAC | BamHI | 312 | ATCC® 14,028™ |
R: GCGCTCGAGAGACTGAACCGCCAGTTC | XhoI | |||
ApxIIPF | F: GCGGATCCATGTCAAAAATCACTTTGTCATCAT | BamHI | 1140 | ATCC® 27088™ |
R: CGCTCGAGAGCTCCAACTCCACCGGAGAT | XhoI | |||
nFliC-gs | F: GGATCCATGGCACAAGTCATTAATACAAAC | BamHI- | 312 | nFliC |
R: GCTGCCGCCCCCGCCAGACTGAACCGCCAGTTC | ||||
ApxIIPF-gs | F: GGCGGGGGCGGCAGCATGTCAAAAATCACTTTGTCATCAT | -XhoI | 1140 | ApxIIPF |
R: CGCTCGAGAGCTCCAACTCCACCGGAGAT | ||||
ApxIIPF-nFliC | F: nFliC F | BamHI XhoI | 1467 | ApxIIPF and nFliC |
R: ApxIIPF R |